Review



gapdh mouse monoclonal antibody sinopept  (Cell Signaling Technology Inc)


Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 96
      Buy from Supplier

    Structured Review

    Cell Signaling Technology Inc gapdh mouse monoclonal antibody sinopept
    Fig. 5. Role of M2 AChR on the levels of ROS in the presence of ACh during H/R. (A) The silencing efficiency of M2 AChR siRNA were detected by Western blotting, equal protein loading was confirmed with the <t>GAPDH</t> antibody. (B) The effects of knockdown of M2 AChR on cell injury were assayed by MTT. (C, D) M2 AChR small siRNA transfection increases the contents of ROS in the presence of ACh during H/R. All the transfection groups were exposed to H/R with ACh followed by DCFH-DA staining 48 h after transfection. Data are presented as means ± SEM. n = 4. **P < 0.01 vs NC siRNA group; ##P < 0.01 vs NC siRNA exposed to HR with ACh group. Scale bar = 50 µm.
    Gapdh Mouse Monoclonal Antibody Sinopept, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 490 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gapdh+mouse+monoclonal+antibody+sinopept/GAPDH+Mouse+mAb/pm23407103-50-11-26
    Average 96 stars, based on 490 article reviews
    gapdh mouse monoclonal antibody sinopept - by Bioz Stars, 2026-11
    96/100 stars

    Images

    1) Product Images from "Acetylcholine attenuates hypoxia/ reoxygenation-induced mitochondrial and cytosolic ROS formation in H9c2 cells via M2 acetylcholine receptor."

    Article Title: Acetylcholine attenuates hypoxia/ reoxygenation-induced mitochondrial and cytosolic ROS formation in H9c2 cells via M2 acetylcholine receptor.

    Journal: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

    doi: 10.1159/000343360

    Fig. 5. Role of M2 AChR on the levels of ROS in the presence of ACh during H/R. (A) The silencing efficiency of M2 AChR siRNA were detected by Western blotting, equal protein loading was confirmed with the GAPDH antibody. (B) The effects of knockdown of M2 AChR on cell injury were assayed by MTT. (C, D) M2 AChR small siRNA transfection increases the contents of ROS in the presence of ACh during H/R. All the transfection groups were exposed to H/R with ACh followed by DCFH-DA staining 48 h after transfection. Data are presented as means ± SEM. n = 4. **P < 0.01 vs NC siRNA group; ##P < 0.01 vs NC siRNA exposed to HR with ACh group. Scale bar = 50 µm.
    Figure Legend Snippet: Fig. 5. Role of M2 AChR on the levels of ROS in the presence of ACh during H/R. (A) The silencing efficiency of M2 AChR siRNA were detected by Western blotting, equal protein loading was confirmed with the GAPDH antibody. (B) The effects of knockdown of M2 AChR on cell injury were assayed by MTT. (C, D) M2 AChR small siRNA transfection increases the contents of ROS in the presence of ACh during H/R. All the transfection groups were exposed to H/R with ACh followed by DCFH-DA staining 48 h after transfection. Data are presented as means ± SEM. n = 4. **P < 0.01 vs NC siRNA group; ##P < 0.01 vs NC siRNA exposed to HR with ACh group. Scale bar = 50 µm.

    Techniques Used: Western Blot, Knockdown, Transfection, Staining

    Related Articles

    Western Blot:

    Article Title: Acetylcholine attenuates hypoxia/ reoxygenation-induced mitochondrial and cytosolic ROS formation in H9c2 cells via M2 acetylcholine receptor.
    Article Snippet: Protein levels were analyzed in whole cell lysates obtained using Cell lysis buffer for Western and IP (Beyotime) and 50 μg of samples were resolved on a SDS polyacrylamide gel. .. Gels were then analyzed by Western blot with the following antibodies: GAPDH Mouse Monoclonal Antibody (Sinopept), mAChR M2 pAb, caspase-3 pAb (Bioworld Technology), rac 1 pAb (Cell Signaling Technology). .. To test the levels of mtROS, MitoSOX Red, a fluorochrome specific to anion superoxide produced in the inner mitochondrial compartment (Invitrogen) was used.



    Similar Products

    gapdh mouse monoclonal antibody sinopept  (Cell Signaling Technology Inc)
    96
    Cell Signaling Technology Inc gapdh mouse monoclonal antibody sinopept
    Fig. 5. Role of M2 AChR on the levels of ROS in the presence of ACh during H/R. (A) The silencing efficiency of M2 AChR siRNA were detected by Western blotting, equal protein loading was confirmed with the <t>GAPDH</t> antibody. (B) The effects of knockdown of M2 AChR on cell injury were assayed by MTT. (C, D) M2 AChR small siRNA transfection increases the contents of ROS in the presence of ACh during H/R. All the transfection groups were exposed to H/R with ACh followed by DCFH-DA staining 48 h after transfection. Data are presented as means ± SEM. n = 4. **P < 0.01 vs NC siRNA group; ##P < 0.01 vs NC siRNA exposed to HR with ACh group. Scale bar = 50 µm.
    Gapdh Mouse Monoclonal Antibody Sinopept, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gapdh+mouse+monoclonal+antibody+sinopept/GAPDH+Mouse+mAb/pm23407103-50-11-26
    Average 96 stars, based on 1 article reviews
    gapdh mouse monoclonal antibody sinopept - by Bioz Stars, 2026-11
    96/100 stars
    		  Buy from Supplier

    Image Search Results


    Fig. 5. Role of M2 AChR on the levels of ROS in the presence of ACh during H/R. (A) The silencing efficiency of M2 AChR siRNA were detected by Western blotting, equal protein loading was confirmed with the GAPDH antibody. (B) The effects of knockdown of M2 AChR on cell injury were assayed by MTT. (C, D) M2 AChR small siRNA transfection increases the contents of ROS in the presence of ACh during H/R. All the transfection groups were exposed to H/R with ACh followed by DCFH-DA staining 48 h after transfection. Data are presented as means ± SEM. n = 4. **P < 0.01 vs NC siRNA group; ##P < 0.01 vs NC siRNA exposed to HR with ACh group. Scale bar = 50 µm.

    Journal: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

    Article Title: Acetylcholine attenuates hypoxia/ reoxygenation-induced mitochondrial and cytosolic ROS formation in H9c2 cells via M2 acetylcholine receptor.

    doi: 10.1159/000343360

    Figure Lengend Snippet: Fig. 5. Role of M2 AChR on the levels of ROS in the presence of ACh during H/R. (A) The silencing efficiency of M2 AChR siRNA were detected by Western blotting, equal protein loading was confirmed with the GAPDH antibody. (B) The effects of knockdown of M2 AChR on cell injury were assayed by MTT. (C, D) M2 AChR small siRNA transfection increases the contents of ROS in the presence of ACh during H/R. All the transfection groups were exposed to H/R with ACh followed by DCFH-DA staining 48 h after transfection. Data are presented as means ± SEM. n = 4. **P < 0.01 vs NC siRNA group; ##P < 0.01 vs NC siRNA exposed to HR with ACh group. Scale bar = 50 µm.

    Article Snippet: Gels were then analyzed by Western blot with the following antibodies: GAPDH Mouse Monoclonal Antibody (Sinopept), mAChR M2 pAb, caspase-3 pAb (Bioworld Technology), rac 1 pAb (Cell Signaling Technology).

    Techniques: Western Blot, Knockdown, Transfection, Staining